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  • Product Name:

    Human Neutrophil gelatinase-associated lipocalin(NGAL)

  • Product English Name:

    Human Neutrophil gelatinase-associated lipocalin(NGAL)

  • Product Abbreviation:
  • Type:
    Renal Function & Injury
  • Code:
  • Alias:
    25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • Description:

    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron,leading to limit bacterial growth.

  • Purity:
    >90% by SDS-PAGE
  • Shape:
    Liquid, dissolved in 0.01M PBS, pH 7.4, 50% glycerol
  • Storage:
    Aliquot and store at -20 ° C or -80 ° C. Avoid repeated freeze / thaw cycles
  • Application:
    Calibrator or standard in ELISA, latex enhanced turbidimetricimmunoassay(LETIA ), chemiluminescent immunoassay (CLIA) and other possible application
  • Active:
    Measurement of the antigen-antibody complex in particle-enhanced turbidimetric immunoassay . The increased degree of the turbidity can be determined turbidimetrically at 546 nm. Protein concentration is determined >1mg/ml by commercial NGAL reagent, Imm
  • Size: